Overview¶
The deGFP Reporter Module produced deGFP, a green fluorescent protein.
Schematic
Designs
A schematic representation of PURE converting template DNA into a fluorescent reporter.
| Name | Length (bp) | File |
|---|---|---|
pOpen-pT7-deGFP | 2500 | path |
Cytosols¶
Reference Composition¶
This composition was evaluated in the DevNote by Yadav (2025)
| Component | Stock Concentration | Final Concentration in Reaction |
|---|---|---|
| PMix | 15 mg/mL | 1.8 mg/mL |
| SMix | 4X | 1X |
| Ribosomes | 10 M | 1.8 M |
| tRNA | varies | 3.5 mg/mL |
| pOpen-deGFP | varies | 3 nM |
| Magnesium acetate | 200 mM | varies |
Expected Behavior¶
Kinetics
Endpoint
Translation kinetics of Cytosol and PURExpress reactions using two different pOpen-deGFP DNA preps. Cytosol w/o DNA refers to the Cytosol reaction lacking the pOpen-deGFP template. Data from Yadav (2025).
Final protein yields of the reactions measured at steady state. Data from Yadav (2025).
Protocols¶
Cells¶
Reference Composition¶
Coming soon!
Expected Behavior¶
Coming soon!
Protocols¶
- Yadav, S. (2025). First Nucleus Cytosol Testing: Evaluation of Nucleus Cytosol v0.5 Through deGFP Expression. 10.63765/fppr8928